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Henan Tongwei Medical Equipment Co., LtdCall us : +86 − −19139704654 // Email us : [email protected]
Henan Tongwei Medical Equipment Co., LtdCall us : +86 − −19139704654 // Email us : [email protected]
THP-1 is a human monocytic leukemia cell line After treatment with phorbol esters THP-1 cells differentiate into macrophage-like cells which mimic native monocyte-derived macrophages in several respects Compared to other human myeloid cell lines such as HL-60 U937 KG-1 or HEL cell lines differentiated THP-1 cells behave more like native monocyte-derived macrophages Because of these
Fig 1 Selection of polyclonal aptamers specific for TIMCs HT Cell-SELEX was performed on MSC2 cells treated with IL4 or left untreated as a proxy of tumor-infiltrating and splenic myeloid cells respectively (A) FACS analysis of IL4-treated MSC2 stained with the Cy3-labeled RNA aptamers from cycle 0 as control or cycle 11
The IL-3–dependent murine myeloid cell line 32Dcl3 (kindly provided by Dr Felicitas Rosenthal Freiburg Germany) was cultured in RPMI 1640 supplemented with 10% WEHI-conditioned medium as a source of IL-3 10% fetal calf serum (FCS) and antibiotics at
2018/9/15To further clarify the function of TCEA1 we analyzed survival/proliferation cell cycle differentiation and apoptosis of myeloid cells after the down-regulation of TCEA1 in vitro using the 32Dcl3 myeloid cell line The 32Dcl3 cells represent a simplified in vitro model
THP-1 is a human monocytic leukemia cell line After treatment with phorbol esters THP-1 cells differentiate into macrophage-like cells which mimic native monocyte-derived macrophages in several respects Compared to other human myeloid cell lines such as HL-60 U937 KG-1 or HEL cell lines differentiated THP-1 cells behave more like native monocyte-derived macrophages Because of these
The interleukin 3(IL‐3)‐dependent murine myeloid cell line 32Dcl3 (kindly provided by Dr Felicitas Rosenthal Freiburg Germany) was cultured in RPMI‐1640 supplemented with 10% WEHI‐conditioned medium as a source of IL‐3 10% fetal calf serum and 2
The IL-3–dependent murine myeloid cell line 32Dcl3 (kindly provided by Dr Felicitas Rosenthal Freiburg Germany) was cultured in RPMI 1640 supplemented with 10% WEHI-conditioned medium as a source of IL-3 10% fetal calf serum (FCS) and antibiotics at
To define the role of p55CDC during the mammalian cell cycle we overexpressed this protein in the murine myeloid cell line 32Dcl3 32Dcl3 cells are an ideal model system because these cells can be induced to proliferate differentiate or activate cellular
The murine Cebpa gene contains an evolutionarily conserved 453 bp enhancer located at +37 kb that together with its promoter directs expression to myeloid progenitors and to long-term hematopoietic stem cells in transgenic mice In human acute myeloid
The IL-3-dependent murine myeloid 32Dcl3 cell line (subsequently referred to as 32D) was obtained from the American Type Culture Collection (Rockville MD) and maintained in RPMI 1640 containing 10% FCS 10% WEHI-conditioned medium (as a source of IL
The IL-3–dependent murine myeloid cell line 32Dcl3 (kindly provided by Dr Felicitas Rosenthal Freiburg Germany) was cultured in RPMI 1640 supplemented with 10% WEHI-conditioned medium as a source of IL-3 10% fetal calf serum (FCS) and antibiotics at
2019/7/4Next we analyzed TNFα gene expression in the murine myeloid progenitor cell line 32D and the murine lymphoid progenitor cell line BA/F3 upon Bcr-Abl expression Both progenitor cell types depend on IL-3 signaling and Bcr-Abl expression induces cytokine-independent growth
Fig 1 Selection of polyclonal aptamers specific for TIMCs HT Cell-SELEX was performed on MSC2 cells treated with IL4 or left untreated as a proxy of tumor-infiltrating and splenic myeloid cells respectively (A) FACS analysis of IL4-treated MSC2 stained with the Cy3-labeled RNA aptamers from cycle 0 as control or cycle 11
The murine myeloid cell line 32Dcl3 is one of the few cell lines that can terminally differentiate into neutrophils Granulocyte colony-stimulating factor (G-CSF) drives the differentiation of these cells therefore G-CSF receptor signaling for neutrophil proliferation and differentiation has been studied extensively using this cell line as a model
32Dcl3 is an IL‐3‐dependent murine granulocytic precursor cell line which can be made to undergo differentiation to mature neutrophils upon replacement of IL‐3 with G‐CSF Removal of the cells from IL‐3 results in apoptosis
2018/9/15The murine myeloid cell line 32Dcl3 as a model system for studying neutrophil functions Journal of Immunological Methods 283:195–204 [] [Google Scholar] 18 Gupta D Shah HP Malu K Berliner N and Gaines P (2014) Differentiation and characterization of
The Rat2 fibroblast cell line was subsequently transduced with CalDAG-GEF Ia-expressing virus or empty vector virus pools and selected in G418 Stable Rat2 transductants were obtained and pools of greater than 50 colonies of each were assayed for growth in soft agar and growth rates in 0 5 or 5% FBS with or without added PMA a diacylglycerol analog were measured
2012/12/6The murine myeloid cell line 32Dcl3 as a model system for studying neutrophil functions J Immunol Methods 2003 283 (1):195–204 [] 24 Rosas M Osorio F Robinson MJ et al Hoxb8 conditionally immortalised macrophage lines model inflammatory Eur J 41
The murine myeloid progenitor cell line 32Dcl3 (2 37 51) was maintained in Iscove's modified Dulbecco's medium supplemented with 10% fetal bovine serum 0 5% penicillin-streptomycin and 10% WEHI-3B cell-conditioned medium as a source of IL-3 ()
2012/12/6The murine myeloid cell line 32Dcl3 as a model system for studying neutrophil functions J Immunol Methods 2003 283 (1):195–204 [] 24 Rosas M Osorio F Robinson MJ et al Hoxb8 conditionally immortalised macrophage lines model inflammatory Eur J 41
2018/9/15The murine myeloid cell line 32Dcl3 as a model system for studying neutrophil functions Journal of Immunological Methods 283:195–204 [] [Google Scholar] 18 Gupta D Shah HP Malu K Berliner N and Gaines P (2014) Differentiation and characterization of
Differentiation of a murine myeloblastic cell line 32Dcl3 cells is detailed in Basic Protocol 4 This cell line demonstrates morphologic maturation that is accompanied by upregulated expression of multiple secondary granule protein genes
To define the role of p55CDC during the mammalian cell cycle we overexpressed this protein in the murine myeloid cell line 32Dcl3 32Dcl3 cells are an ideal model system because these cells can be induced to proliferate differentiate or activate cellular
The IL-3–dependent murine myeloid cell line 32Dcl3 (kindly provided by Dr Felicitas Rosenthal Freiburg Germany) was cultured in RPMI 1640 supplemented with 10% WEHI-conditioned medium as a source of IL-3 10% fetal calf serum (FCS) and antibiotics at
32Dcl3 is an IL‐3‐dependent murine granulocytic precursor cell line which can be made to undergo differentiation to mature neutrophils upon replacement of IL‐3 with G‐CSF Removal of the cells from IL‐3 results in apoptosis
Cells The murine IL-3–dependent 32Dcl3 cell line 28 (a gift of Dr Alan D Friedman Johns Hopkins University) was maintained in Iscove-modified Dulbecco medium (Mediatech Cellgro Herndon VA) containing 10% heat-inactivated fetal bovine serum (FBS Biosource Camarillo CA) plus 100 pg/mL IL-3 (PeproTech Rocky Hill NJ) with biweekly subculture
This murine model will serve as a powerful tool to identify the spectrum of mutations that cooperate with AML1-ETO in leukemogenesis Estrogen-dependent E2a/Pbx1 myeloid cell line exhibit conditional differentiation that can be arrested by other leukemic 98
32Dcl3 is an IL‐3‐dependent murine granulocytic precursor cell line which can be made to undergo differentiation to mature neutrophils upon replacement of IL‐3 with G‐CSF Removal of the cells from IL‐3 results in apoptosis
The IL-3–dependent murine myeloid cell line 32Dcl3 (kindly provided by Dr Felicitas Rosenthal Freiburg Germany) was cultured in RPMI 1640 supplemented with 10% WEHI-conditioned medium as a source of IL-3 10% fetal calf serum (FCS) and antibiotics at
The murine myeloid cell line 32Dcl3 is one of the few cell lines that can terminally differentiate into neutrophils Granulocyte colony-stimulating factor (G-CSF) drives the differentiation of these cells therefore G-CSF receptor signaling for neutrophil proliferation and differentiation has been studied extensively using this cell line as a model
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