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the murine myeloid cell line 32dcl3 as a model

  • The human leukemia cell line THP

    THP-1 is a human monocytic leukemia cell line After treatment with phorbol esters THP-1 cells differentiate into macrophage-like cells which mimic native monocyte-derived macrophages in several respects Compared to other human myeloid cell lines such as HL-60 U937 KG-1 or HEL cell lines differentiated THP-1 cells behave more like native monocyte-derived macrophages Because of these

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  • Aptamers against mouse and human tumor

    Fig 1 Selection of polyclonal aptamers specific for TIMCs HT Cell-SELEX was performed on MSC2 cells treated with IL4 or left untreated as a proxy of tumor-infiltrating and splenic myeloid cells respectively (A) FACS analysis of IL4-treated MSC2 stained with the Cy3-labeled RNA aptamers from cycle 0 as control or cycle 11

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  • Flt3 mutations from patients with acute myeloid leukemia

    The IL-3–dependent murine myeloid cell line 32Dcl3 (kindly provided by Dr Felicitas Rosenthal Freiburg Germany) was cultured in RPMI 1640 supplemented with 10% WEHI-conditioned medium as a source of IL-3 10% fetal calf serum (FCS) and antibiotics at

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  • TCEA1 regulates the proliferative potential of mouse

    2018/9/15To further clarify the function of TCEA1 we analyzed survival/proliferation cell cycle differentiation and apoptosis of myeloid cells after the down-regulation of TCEA1 in vitro using the 32Dcl3 myeloid cell line The 32Dcl3 cells represent a simplified in vitro model

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  • The human leukemia cell line THP

    THP-1 is a human monocytic leukemia cell line After treatment with phorbol esters THP-1 cells differentiate into macrophage-like cells which mimic native monocyte-derived macrophages in several respects Compared to other human myeloid cell lines such as HL-60 U937 KG-1 or HEL cell lines differentiated THP-1 cells behave more like native monocyte-derived macrophages Because of these

    Get Price
  • Constitutive activation of FLT3 in acute myeloid leukaemia

    The interleukin 3(IL‐3)‐dependent murine myeloid cell line 32Dcl3 (kindly provided by Dr Felicitas Rosenthal Freiburg Germany) was cultured in RPMI‐1640 supplemented with 10% WEHI‐conditioned medium as a source of IL‐3 10% fetal calf serum and 2

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  • Flt3 mutations from patients with acute myeloid leukemia

    The IL-3–dependent murine myeloid cell line 32Dcl3 (kindly provided by Dr Felicitas Rosenthal Freiburg Germany) was cultured in RPMI 1640 supplemented with 10% WEHI-conditioned medium as a source of IL-3 10% fetal calf serum (FCS) and antibiotics at

    Get Price
  • The role of p55CDC in cell cycle control and mammalian

    To define the role of p55CDC during the mammalian cell cycle we overexpressed this protein in the murine myeloid cell line 32Dcl3 32Dcl3 cells are an ideal model system because these cells can be induced to proliferate differentiate or activate cellular

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  • The +37 Kb Cebpa Enhancer Is Critical for Cebpa Myeloid

    The murine Cebpa gene contains an evolutionarily conserved 453 bp enhancer located at +37 kb that together with its promoter directs expression to myeloid progenitors and to long-term hematopoietic stem cells in transgenic mice In human acute myeloid

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  • Constitutive Activation of Akt by Flt3 Internal Tandem

    The IL-3-dependent murine myeloid 32Dcl3 cell line (subsequently referred to as 32D) was obtained from the American Type Culture Collection (Rockville MD) and maintained in RPMI 1640 containing 10% FCS 10% WEHI-conditioned medium (as a source of IL

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  • Flt3 mutations from patients with acute myeloid leukemia

    The IL-3–dependent murine myeloid cell line 32Dcl3 (kindly provided by Dr Felicitas Rosenthal Freiburg Germany) was cultured in RPMI 1640 supplemented with 10% WEHI-conditioned medium as a source of IL-3 10% fetal calf serum (FCS) and antibiotics at

    Get Price
  • Infliximab therapy together with tyrosine kinase inhibition

    2019/7/4Next we analyzed TNFα gene expression in the murine myeloid progenitor cell line 32D and the murine lymphoid progenitor cell line BA/F3 upon Bcr-Abl expression Both progenitor cell types depend on IL-3 signaling and Bcr-Abl expression induces cytokine-independent growth

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  • Aptamers against mouse and human tumor

    Fig 1 Selection of polyclonal aptamers specific for TIMCs HT Cell-SELEX was performed on MSC2 cells treated with IL4 or left untreated as a proxy of tumor-infiltrating and splenic myeloid cells respectively (A) FACS analysis of IL4-treated MSC2 stained with the Cy3-labeled RNA aptamers from cycle 0 as control or cycle 11

    Get Price
  • The murine myeloid cell line 32Dcl3 as a model system for

    The murine myeloid cell line 32Dcl3 is one of the few cell lines that can terminally differentiate into neutrophils Granulocyte colony-stimulating factor (G-CSF) drives the differentiation of these cells therefore G-CSF receptor signaling for neutrophil proliferation and differentiation has been studied extensively using this cell line as a model

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  • p120 nucleolar‐proliferating antigen is a direct target of

    32Dcl3 is an IL‐3‐dependent murine granulocytic precursor cell line which can be made to undergo differentiation to mature neutrophils upon replacement of IL‐3 with G‐CSF Removal of the cells from IL‐3 results in apoptosis

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  • TCEA1 regulates the proliferative potential of mouse

    2018/9/15The murine myeloid cell line 32Dcl3 as a model system for studying neutrophil functions Journal of Immunological Methods 283:195–204 [] [Google Scholar] 18 Gupta D Shah HP Malu K Berliner N and Gaines P (2014) Differentiation and characterization of

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  • Activation of the Rap1 Guanine Nucleotide Exchange

    The Rat2 fibroblast cell line was subsequently transduced with CalDAG-GEF Ia-expressing virus or empty vector virus pools and selected in G418 Stable Rat2 transductants were obtained and pools of greater than 50 colonies of each were assayed for growth in soft agar and growth rates in 0 5 or 5% FBS with or without added PMA a diacylglycerol analog were measured

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  • Identification of key regulatory pathways of myeloid

    2012/12/6The murine myeloid cell line 32Dcl3 as a model system for studying neutrophil functions J Immunol Methods 2003 283 (1):195–204 [] 24 Rosas M Osorio F Robinson MJ et al Hoxb8 conditionally immortalised macrophage lines model inflammatory Eur J 41

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  • Molecular Mechanisms Associated with the Regulation of

    The murine myeloid progenitor cell line 32Dcl3 (2 37 51) was maintained in Iscove's modified Dulbecco's medium supplemented with 10% fetal bovine serum 0 5% penicillin-streptomycin and 10% WEHI-3B cell-conditioned medium as a source of IL-3 ()

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  • Identification of key regulatory pathways of myeloid

    2012/12/6The murine myeloid cell line 32Dcl3 as a model system for studying neutrophil functions J Immunol Methods 2003 283 (1):195–204 [] 24 Rosas M Osorio F Robinson MJ et al Hoxb8 conditionally immortalised macrophage lines model inflammatory Eur J 41

    Get Price
  • TCEA1 regulates the proliferative potential of mouse

    2018/9/15The murine myeloid cell line 32Dcl3 as a model system for studying neutrophil functions Journal of Immunological Methods 283:195–204 [] [Google Scholar] 18 Gupta D Shah HP Malu K Berliner N and Gaines P (2014) Differentiation and characterization of

    Get Price
  • Differentiation and Characterization of Myeloid Cells

    Differentiation of a murine myeloblastic cell line 32Dcl3 cells is detailed in Basic Protocol 4 This cell line demonstrates morphologic maturation that is accompanied by upregulated expression of multiple secondary granule protein genes

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  • The role of p55CDC in cell cycle control and mammalian

    To define the role of p55CDC during the mammalian cell cycle we overexpressed this protein in the murine myeloid cell line 32Dcl3 32Dcl3 cells are an ideal model system because these cells can be induced to proliferate differentiate or activate cellular

    Get Price
  • Flt3 mutations from patients with acute myeloid leukemia

    The IL-3–dependent murine myeloid cell line 32Dcl3 (kindly provided by Dr Felicitas Rosenthal Freiburg Germany) was cultured in RPMI 1640 supplemented with 10% WEHI-conditioned medium as a source of IL-3 10% fetal calf serum (FCS) and antibiotics at

    Get Price
  • p120 nucleolar‐proliferating antigen is a direct target of

    32Dcl3 is an IL‐3‐dependent murine granulocytic precursor cell line which can be made to undergo differentiation to mature neutrophils upon replacement of IL‐3 with G‐CSF Removal of the cells from IL‐3 results in apoptosis

    Get Price
  • Transcription factor Gfi

    Cells The murine IL-3–dependent 32Dcl3 cell line 28 (a gift of Dr Alan D Friedman Johns Hopkins University) was maintained in Iscove-modified Dulbecco medium (Mediatech Cellgro Herndon VA) containing 10% heat-inactivated fetal bovine serum (FBS Biosource Camarillo CA) plus 100 pg/mL IL-3 (PeproTech Rocky Hill NJ) with biweekly subculture

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  • Expression of a conditional AML1

    This murine model will serve as a powerful tool to identify the spectrum of mutations that cooperate with AML1-ETO in leukemogenesis Estrogen-dependent E2a/Pbx1 myeloid cell line exhibit conditional differentiation that can be arrested by other leukemic 98

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  • p120 nucleolar‐proliferating antigen is a direct target of

    32Dcl3 is an IL‐3‐dependent murine granulocytic precursor cell line which can be made to undergo differentiation to mature neutrophils upon replacement of IL‐3 with G‐CSF Removal of the cells from IL‐3 results in apoptosis

    Get Price
  • Flt3 mutations from patients with acute myeloid leukemia

    The IL-3–dependent murine myeloid cell line 32Dcl3 (kindly provided by Dr Felicitas Rosenthal Freiburg Germany) was cultured in RPMI 1640 supplemented with 10% WEHI-conditioned medium as a source of IL-3 10% fetal calf serum (FCS) and antibiotics at

    Get Price
  • The murine myeloid cell line 32Dcl3 as a model system for

    The murine myeloid cell line 32Dcl3 is one of the few cell lines that can terminally differentiate into neutrophils Granulocyte colony-stimulating factor (G-CSF) drives the differentiation of these cells therefore G-CSF receptor signaling for neutrophil proliferation and differentiation has been studied extensively using this cell line as a model

    Get Price
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Henan Tongwei

Henan Tongwei Medical Device Co., Ltd. is a branch company of Guangzhou Ningwei Technology Co., LTD., which is specialized in the production, processing, sales, research and development and service of related products and equipment in the medical device industry.

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